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RUVBL2 (NM_006666) Human Over-expression Lysate

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SKU
LY401993
Transient overexpression lysate of RuvB-like 2 (E. coli) (RUVBL2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200933
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006666, NP_006657
Synonyms CGI-46; ECP-51; ECP51; INO80J; REPTIN; RVB2; TAP54-beta; TIH2; TIP48; TIP49B
Predicted MW 51.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_006657
Locus ID 10856
Cytogenetics 19q13.33
Protein Families WB
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Other "RUVBL2" proteins (3)
SKU Description Size Price
PH300933 RUVBL2 MS Standard C13 and N15-labeled recombinant protein (NP_006657) 10 ug
$3,360.00
LC401993 Lyophilized RUVBL2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP300933 Recombinant protein of human RuvB-like 2 (E. coli) (RUVBL2), 20 µg 20 ug
$737.00

file-alt Citations

Removal of H2A.Z by INO80 promotes homologous recombination
Alatwi, Downs
EMBO Rep. (2015) 16 (8), 986-94 DOI: 10.15252/embr.201540330
Cited by 146
AI Summary: In this study, the OriGene product pEGFP-RUVBL2 was used to transfect human U2OS cells, enabling the investigation of the role of INO80 and H2A.Z removal in promoting homologous recombination.
Snippet: Exponentially growing human U2OS cells were plated onto 35-mm glass-bottom dishes (MatTek) and transfected with the pTGFP-H2AZ construct, pEGFP-RUVBL2 (Origene) or pEGFP-H2B using NanoJuice according to the manufacturer’s protocol. The cells were allowed to express the construct for 24 h and were then incubated
Showing 1-1 of 1 papers.
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