MCM7 (NM_005916) Human Over-expression Lysate
SKU
LY401787
Transient overexpression lysate of minichromosome maintenance complex component 7 (MCM7), transcript variant 1 (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC213809 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_005916, NP_005907 |
| Synonyms | CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104 |
| Predicted MW | 81.1 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_005907 |
| Locus ID | 4176 |
| Cytogenetics | 7q22.1 |
| Protein Families | WB |
| Protein Pathways | Cell cycle, DNA replication |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "MCM7" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH313809 | MCM7 MS Standard C13 and N15-labeled recombinant protein (NP_005907) | 10 ug |
$3,360.00
|
|
| LC401787 | Lyophilized MCM7 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP313809 | Recombinant protein of human minichromosome maintenance complex component 7 (MCM7), transcript variant 1, 20 µg | 20 ug |
$737.00
|
|
Citations
Homozygous mutation in MCM7 causes autosomal recessive primary microcephaly and intellectual disability
Ravindran, Gutierrez de Velazco, Ghazanfar et al
J Med Genet (2021)
Ravindran, Gutierrez de Velazco, Ghazanfar et al
J Med Genet (2021)
Cited by 7
AI Summary: OriGene’s human WT-MCM7 and Mut-MCM7 overexpression plasmids were used to generate stable cell lines, enabling the authors to demonstrate that only wild-type MCM7 could rescue cell proliferation defects caused by Mcm7 downregulation, thereby supporting the disease-causative role of the identified mutation.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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