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Adiponectin (ADIPOQ) (NM_004797) Human Over-expression Lysate

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SKU
LY401510
Transient overexpression lysate of adiponectin, C1Q and collagen domain containing (ADIPOQ) (as WB positive control)
  $436.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215161
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004797, NP_004788
Synonyms ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
Predicted MW 26.41 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004788
Locus ID 9370
Cytogenetics 3q27.3
Protein Families WB
Protein Pathways Adipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus
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Other "Adiponectin" proteins (10)
SKU Description Size Price
PH315161 ADIPOQ MS Standard C13 and N15-labeled recombinant protein (NP_004788) 10 ug
$3,360.00
LC401510 Lyophilized ADIPOQ HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC432756 Lyophilized ADIPOQ HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY432756 Transient overexpression lysate of adiponectin, C1Q and collagen domain containing (ADIPOQ), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP315161 Recombinant protein of human adiponectin, C1Q and collagen domain containing (ADIPOQ), 20 µg 20 ug
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TP700247 Purified secreted form of human adiponectin, C1Q and collagen domain containing (ADIPOQ), with N-terminal His tag, expressed in human cells, 20ug 20 ug
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TP710245 Purified recombinant protein of Human adiponectin, C1Q and collagen domain containing (ADIPOQ), transcript variant 2, full length, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP710246 Purified recombinant protein of Human adiponectin, C1Q and collagen domain containing (ADIPOQ), transcript variant 2, residues 18-244aa, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
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TP720685 Purified recombinant protein of Human adiponectin, C1Q and collagen domain containing (ADIPOQ), transcript variant 2 10 ug
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TP790074 Purified recombinant protein of Human adiponectin, C1Q and collagen domain containing (ADIPOQ), transcript variant 2, esidues 19-244aa, with C-terminal DDK tag, secretory expressed in CHO cells, 50ug 50 ug
$540.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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