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TRAF6 (NM_004620) Human Over-expression Lysate

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SKU
LY401463
Transient overexpression lysate of TNF receptor-associated factor 6 (TRAF6), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219528
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004620, NP_004611
Synonyms MGC:3310; RNF85
Predicted MW 59.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004611
Locus ID 7189
Cytogenetics 11p12
Protein Families WB
Protein Pathways Endocytosis, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, Toll-like receptor signaling pathway, Ubiquitin mediated proteolysis
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Other "TRAF6" proteins (5)
SKU Description Size Price
PH319528 TRAF6 MS Standard C13 and N15-labeled recombinant protein (NP_004611) 10 ug
$3,360.00
LC401463 Lyophilized TRAF6 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC403440 Lyophilized TRAF6 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403440 Transient overexpression lysate of TNF receptor-associated factor 6 (TRAF6), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP319528 Recombinant protein of human TNF receptor-associated factor 6 (TRAF6), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

TAOK1 positively regulates TLR4-induced inflammatory responses by promoting ERK1/2 activation in macrophages
Zhu, Yu, Gao et al
Mol. Immunol. (2020) 122, 124-131 DOI: 10.1016/j.molimm.2020.04.006
Cited by 16
AI Summary: In this study, OriGene products—including anti-Myc, anti-DDK(Flag), anti-HA antibodies, and Flag-TRAF6—were used to detect and analyze protein expression and interactions critical to elucidating the role of TAOK1 in TLR4-induced inflammatory responses in macrophages.
Snippet: Anti-TAOK1, anti-TRAF3, anti-TRAF6, anti-TPL2, anti-β-actin, and HRP-coupled secondary antibodies were from Santa Cruz. Anti-Myc, anti-DDK(Flag), anti-HA were from Origene Flag-TRAF6 was purchased from Origene;- Anti-TAOK1, anti-TRAF3, anti-TRAF6, anti-TPL2, anti-β-actin, and HRP-coupled secondary antibodies were from Santa Cruz. Anti-Myc, anti-DDK(Flag), anti-HA were from Origene Flag-TRAF6 was purchased from Origene
TRAF6 function as a novel co-regulator of Wnt3a target genes in prostate cancer
Aripaka, Gudey, Zang et al
EBioMedicine (2019) 45, 192-207 DOI: 10.1016/j.ebiom.2019.06.046
AI Summary: In this study, recombinant human TRAF6-Flag protein from OriGene (#TP319528) was used to investigate the role of TRAF6 in Wnt3a signaling and its regulation of Wnt target genes in prostate cancer.
Snippet: … Expression of LRP51407–1615HA-(His)6 WT was performed in human embryonic kidney (HEK293) cells and purified by Ni-NTA chromatography. Recombinant human TRAF6-Flag was purchased from Origene, USA (#:TP319528). 2.6 Dual luciferase assay Wnt signaling was investigated by TCF reporter plasmid TOPflash transiently transfected into PC3U and SW480 cell lines along with either siTRAF6 o;... … Expression of LRP51407–1615HA-(His)6 WT was performed in human embryonic kidney (HEK293) cells and purified by Ni-NTA chromatography. Recombinant human TRAF6-Flag was purchased from Origene, USA (#:TP319528). 2.6 Dual luciferase assay Wnt signaling was investigated by TCF reporter plasmid TOPflash transiently transfected into PC3U and SW480 cell lines along with either siTRAF6 o;Expression of LRP5 1407-1615 HA-(His) 6 WT was performed in human embryonic kidney (HEK293) cells and purified by Ni-NTA chromatography. Recombinant human TRAF6-Flag was purchased from Origene, USA (#:TP319528). 2.6. …A synthetic gene encoding for the intracellular domain of human LRP5 from amino acid 1407 until 1615 was ordered from Eurofinsgenomics (Ebersberg, Germany) as wild type LRP51407–1615WT or with a mutant TRAF6 consensus binding motif LRP51407–1615 E1425A and further cloned into the eukaryotic expression vector pCMVtag5 upstream of the HA and (His)6 tag. Expression of LRP51407–1615HA-(His)6 WT was performed in human embryonic kidney (HEK293) cells and purified by Ni-NTA chromatography. Recombinant human TRAF6-Flag was purchased from Origene, USA (#:TP319528)
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