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Eg5 (KIF11) (NM_004523) Human Over-expression Lysate

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SKU
LY401441
Transient overexpression lysate of kinesin family member 11 (KIF11) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218842
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004523, NP_004514
Synonyms EG5; HKSP; KNSL1; MCLMR; TRIP5
Predicted MW 119 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004514
Locus ID 3832
Cytogenetics 10q23.33
Protein Families WB
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Other "Eg5" proteins (3)
SKU Description Size Price
PH318842 KIF11 MS Standard C13 and N15-labeled recombinant protein (NP_004514) 10 ug
$3,360.00
LC401441 Lyophilized KIF11 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP318842 Recombinant protein of human kinesin family member 11 (KIF11), 20 µg 20 ug
$867.00

file-alt Citations

Circular RNA‑MTO1 suppresses breast cancer cell viability and reverses monastrol resistance through regulating the TRAF4/Eg5 axis
Liu, Dong, Zhao et al
Int. J. Oncol. (2018)
AI Summary: In this study, the Eg5 overexpression vector from OriGene Technologies, Inc. was used to investigate the role of Eg5 in breast cancer cell viability and monastrol resistance.
Snippet: The circRNA-MTO1 overexpression plasmid was synthesized by Shanghai GenePharma Co., Ltd (Shanghai, China) and an empty vector used as a control. The Eg5 overexpression vector was purchased from OriGene Technologies, Inc
Showing 1-1 of 1 papers.
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