FGF5 (NM_004464) Human Over-expression Lysate
SKU
LY401420
Transient overexpression lysate of fibroblast growth factor 5 (FGF5), transcript variant 1 (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC223402 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_004464, NP_004455 |
| Synonyms | HBGF-5; Smag-82; TCMGLY |
| Predicted MW | 29.55 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_004455 |
| Locus ID | 2250 |
| Cytogenetics | 4q21.21 |
| Protein Families | WB |
| Protein Pathways | MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "FGF5" proteins (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC401420 | Lyophilized FGF5 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC409708 | Lyophilized FGF5 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY409708 | Transient overexpression lysate of fibroblast growth factor 5 (FGF5), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| TP762341 | Purified recombinant protein of Human fibroblast growth factor 5 (FGF5), transcript variant 1, Gly22-End, with N-terminal His tag, expressed in E.coli, 50ug | 50 ug |
$260.00
|
|
Citations
Validation of MicroRNA-188-5p Inhibition Power on Tumor Cell Proliferation in Papillary Thyroid Carcinoma
Zhou, Irving, Wu et al
Cell Transplant (2020) 29, 963689720918300 DOI: 10.1177/0963689720918300
Zhou, Irving, Wu et al
Cell Transplant (2020) 29, 963689720918300 DOI: 10.1177/0963689720918300
Cited by 8
AI Summary: OriGene’s FGF5 3′ UTR luciferase reporter construct was used in this study to experimentally confirm that FGF5 is a direct target of miR-188-5p in papillary thyroid carcinoma cells.
Snippet: Given the crucial role of microRNAs in the cellular proliferation of various types of cancers, we aimed to analyze the expression and function of a cellular proliferation-associated miR-188-5p in p...;The putative targeting gene (FGF5) of miR-188-5p was predicted by TargetScan (http://www.targetscan.org). The 3′ UTR of FGF5 luciferase reporter was commercially available at ORIGENE (Rockville, MD, USA). The cells (TPC-1 and K1) were seeded in a six-well plate (Thermo Fisher Scientific) at a density of 0.25 M cells/well with the complete media for 24 h. Then, cells were cotransfected with 1 µg FGF5 3′-UTR luciferase reporter construct with 20 nM miR-188-5p mimic or miR-NC using lipofectamine 2000 (Invitrogen) with Opti-MEN (Gibco, Shanghai, China) for 48 h. Luciferase assays were performed using the dual-luciferase reporter assay reagent from GeneCopoeia (Rockville, MD, USA). …
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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