EMX2 (NM_004098) Human Over-expression Lysate
SKU
LY401323
Transient overexpression lysate of empty spiracles homeobox 2 (EMX2), transcript variant 1 (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC222758 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_004098, NP_004089 |
| Predicted MW | 28.1 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_004089 |
| Locus ID | 2018 |
| Cytogenetics | 10q26.11 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "EMX2" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC401323 | Lyophilized EMX2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC431133 | Lyophilized EMX2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY431133 | Transient overexpression lysate of empty spiracles homeobox 2 (EMX2), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
Citations
The expression of EMX2 lead to cell cycle arrest in glioblastoma cell line
Monnier, Boniface, Bouvet et al
BMC Cancer (2018) 18 (1), 1213 DOI: 10.1186/s12885-018-5094-y
Monnier, Boniface, Bouvet et al
BMC Cancer (2018) 18 (1), 1213 DOI: 10.1186/s12885-018-5094-y
AI Summary: In this study, the pCMV6-XL5/EMX2 vector from OriGene was used as the source for cloning EMX2, enabling the researchers to generate EMX2-expressing glioblastoma cell lines to investigate the gene's role in cell cycle arrest.
Snippet: Construction of EMX2 expression systems. pcDNA3.1/EMX2 (NM_004098) mammalian expression-vector was sub cloned from pCMV6-XL5/EMX2 vector (Origene). pcDNA3.1/EMX2 and empty vector transfections were performed using Lipofectamine 2000 Cached;pcDNA3.1/EMX2 (NM_004098) mammalian expression-vector was sub cloned from pCMV6-XL5/EMX2 vector (Origene). pcDNA3.1/EMX2 and empty vector transfections were performed using Lipofectamine 2000 (cat. …
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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