OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Nck beta (NCK2) (NM_003581) Human Over-expression Lysate

Be the first to review this product
SKU
LY401190
Transient overexpression lysate of NCK adaptor protein 2 (NCK2), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
Bulk/Customize

Frequently Bought Together (3)

TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA811057 NCK2 mouse monoclonal antibody,clone OTI4H7 100 ul
$447.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "Nck beta or NM_003581"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202557
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003581, NP_003572
Synonyms GRB4; NCKbeta
Predicted MW 42.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003572
Locus ID 8440
Cytogenetics 2q12.2
Protein Families WB
Protein Pathways Axon guidance, ErbB signaling pathway, Pathogenic Escherichia coli infection, T cell receptor signaling pathway
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "Nck beta" proteins (7)
SKU Description Size Price
PH300449 NCK2 MS Standard C13 and N15-labeled recombinant protein (NP_001004720) 10 ug
$3,360.00
PH302557 NCK2 MS Standard C13 and N15-labeled recombinant protein (NP_003572) 10 ug
$3,360.00
LC401190 Lyophilized NCK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423904 Lyophilized NCK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY423904 Transient overexpression lysate of NCK adaptor protein 2 (NCK2), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP300449 Recombinant protein of human NCK adaptor protein 2 (NCK2), transcript variant 2, 20 µg 20 ug
$867.00
TP302557 Recombinant protein of human NCK adaptor protein 2 (NCK2), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products