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Stromal interaction molecule 1 (STIM1) (NM_003156) Human Over-expression Lysate

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SKU
LY401099
Transient overexpression lysate of stromal interaction molecule 1 (STIM1) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205678
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003156, NP_003147
Synonyms D11S4896E; GOK; IMD10; STRMK; TAM; TAM1
Predicted MW 77.42 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003147
Locus ID 6786
Cytogenetics 11p15.4
Protein Families WB
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Other "Stromal interaction molecule 1" proteins (3)
SKU Description Size Price
PH305678 STIM1 MS Standard C13 and N15-labeled recombinant protein (NP_003147) 10 ug
$3,360.00
LC401099 Lyophilized STIM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP305678 Recombinant protein of human stromal interaction molecule 1 (STIM1), 20 µg 20 ug
$867.00

file-alt Citations

ORAI1 Mutations with Distinct Channel Gating Defects in Tubular Aggregate Myopathy
Böhm, Bulla, Urquhart et al
Hum. Mutat. (2017)
AI Summary: In this study, the untagged STIM1 plasmid from OriGene (NM_003156) was used to investigate the functional effects of ORAI1 mutations on calcium channel activity in tubular aggregate myopathy.
Snippet: mutagenesis using the Pfu DNA polymerase (Stratagene, La Jolla, USA). The plasmid carrying an untagged STIM1 was purchased from OriGene (NM_003156;OriGene Technologies, Rockville, USA), and the control vector pEGFP-C1 from Clontech (Palo Alto, CA).
Showing 1-1 of 1 papers.
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