SPG7 (NM_003119) Human Over-expression Lysate
SKU
LY401085
Transient overexpression lysate of spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1 (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC206340 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_003119, NP_003110 |
| Synonyms | CAR; CMAR; PGN; SPG5C |
| Predicted MW | 88.2 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_003110 |
| Locus ID | 6687 |
| Cytogenetics | 16q24.3 |
| Protein Families | WB |
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Resources
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Other "SPG7" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC401085 | Lyophilized SPG7 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP762130 | Purified recombinant protein of Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1,Gly300-Phe573, with N-terminal His tag, expressed in E. coli, 50ug | 50 ug |
$270.00
|
|
| TP762613 | Purified recombinant protein of Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal GST and C-terminal His tag, expressed in E.coli, 50ug | 50 ug |
$270.00
|
|
Citations
Mitochondrial Function in Hereditary Spastic Paraplegia: Deficits in SPG7 but Not SPAST Patient-Derived Stem Cells
Wali, Kumar, Liyanage et al
Front Neurosci (2020) 14, 820 DOI: 10.3389/fnins.2020.00820
Wali, Kumar, Liyanage et al
Front Neurosci (2020) 14, 820 DOI: 10.3389/fnins.2020.00820
Cited by 37
AI Summary: OriGene products contributed to this study by providing the anti-SPG7 antibody (OriGene, TA301533) used for quantifying paraplegin expression in patient-derived stem cells.
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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