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CXCL5 (NM_002994) Human Over-expression Lysate

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SKU
LY401049
Transient overexpression lysate of chemokine (C-X-C motif) ligand 5 (CXCL5) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202707
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002994, NP_002985
Synonyms ENA-78; SCYB5
Predicted MW 12 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002985
Locus ID 6374
Cytogenetics 4q13.3
Protein Families WB
Protein Pathways Chemokine signaling pathway, Cytokine-cytokine receptor interaction
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Other "CXCL5" proteins (7)
SKU Description Size Price
PH302707 CXCL5 MS Standard C13 and N15-labeled recombinant protein (NP_002985) 10 ug
$3,360.00
LC401049 Lyophilized CXCL5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302707 Recombinant protein of human chemokine (C-X-C motif) ligand 5 (CXCL5), 20 µg 20 ug
$867.00
TP700192 Recombinant protein of human chemokine (C-X-C motif) ligand 5 (CXCL5), R45K mutant, 20 ug 20 ug
$900.00
TP700193 Recombinant protein of human chemokine (C-X-C motif) ligand 5 (CXCL5), R48K mutant, 20 ug 20 ug
$900.00
TP700194 Recombinant protein of human chemokine (C-X-C motif) ligand 5 (CXCL5), R45K and R48K double mutant, 20 ug 20 ug
$900.00
TP720876 Purified recombinant protein of Human chemokine (C-X-C motif) ligand 5 (CXCL5) 10 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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