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PSMD13 (NM_002817) Human Over-expression Lysate

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SKU
LY400998
Transient overexpression lysate of proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200037
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002817, NP_002808
Synonyms HSPC027; p40.5; Rpn9; S11
Predicted MW 42.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002808
Locus ID 5719
Cytogenetics 11p15.5
Protein Families WB
Protein Pathways Proteasome
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Other "PSMD13" proteins (5)
SKU Description Size Price
PH300037 PSMD13 MS Standard C13 and N15-labeled recombinant protein (NP_002808) 10 ug
$3,360.00
LC400998 Lyophilized PSMD13 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC406216 Lyophilized PSMD13 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY406216 Transient overexpression lysate of proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP300037 Recombinant protein of human proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Rescue of enzymatic function for disease-associated RPE65s containing various missense mutations in non-active sites
Li, Izumi, Hu et al
J. Biol. Chem. (2014)
AI Summary: OriGene products, specifically PSMD13 siRNA and scramble siRNA, were used in this study to knock down PSMD13 expression and serve as negative controls, enabling the investigation of PSMD13's role in the degradation of mutant RPE65 proteins associated with retinal degeneration.
Snippet: Knockdown of PSMD13- PSMD13 depletion in 293T-LC and ARPE-19 cells was performed by transfecting PSMD13 siRNA (OriGene Technologies, Inc.) using the PolyJet transfection Page 4. 4 ... Scramble siRNA (OriGene Technologies, Inc.) was used as a negative ... Cite SaveSaving
Showing 1-1 of 1 papers.
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