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MC1 Receptor (MC1R) (NM_002386) Human Over-expression Lysate

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SKU
LY400853
Transient overexpression lysate of melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) (MC1R) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203218
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002386, NP_002377
Synonyms CMM5; MSH-R; SHEP2
Predicted MW 34.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002377
Locus ID 4157
Cytogenetics 16q24.3
Protein Families WB
Protein Pathways Melanogenesis, Neuroactive ligand-receptor interaction
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Other "MC1 Receptor" proteins (1)
SKU Description Size Price
LC400853 Lyophilized MC1R HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Melanocortin-1 Receptor Positively Regulates Human Artery Endothelial Cell Migration
Saporiti, Piacentini, Alfieri et al
Cell. Physiol. Biochem. (2019) 52 (6), 1339-1360 DOI: 10.33594/000000094
AI Summary: OriGene’s MC1R full-length cDNA with a C-terminal tGFP tag in the pCMV6 vector was used to transiently transfect HEK293 cells, providing a positive control for detecting MC1R protein in this study.
Snippet: binding of the anti-MC1R antibody to the MC1R protein, we generated a positive control for subsequent analyses by transiently transfecting human HEK293 cells with the MC1R full-length cDNA by a C-terminal fusion of tGFP tag in a pCMV6 vector (Origene, Rockville View as HTML
WB IHC Array
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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