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GBP1 (NM_002053) Human Over-expression Lysate

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SKU
LY400751
Transient overexpression lysate of guanylate binding protein 1, interferon-inducible, 67kDa (GBP1) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201236
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002053, NP_002044
Predicted MW 67.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002044
Locus ID 2633
Cytogenetics 1p22.2
Protein Families WB
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Other "GBP1" proteins (3)
SKU Description Size Price
PH301236 GBP1 MS Standard C13 and N15-labeled recombinant protein (NP_002044) 10 ug
$3,360.00
LC400751 Lyophilized GBP1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP301236 Recombinant protein of human guanylate binding protein 1, interferon-inducible, 67kDa (GBP1), 20 µg 20 ug
$867.00

file-alt Citations

Human guanylate binding proteins potentiate the anti-chlamydia effects of interferon-gamma
Tietzel, El-Haibi, Carabeo
PLoS One (2009) 4 (8), e6499 DOI: 10.1371/journal.pone.0006499
Cited by 103
AI Summary: OriGene products, specifically the hGBP1 and hGBP2 expression plasmids, were used in this study to investigate the roles of human guanylate binding proteins in enhancing the anti-chlamydial effects of interferon-gamma.
FigureFigureFigure
Showing 1-1 of 1 papers.
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