GAP43 (NM_002045) Human Over-expression Lysate
SKU
LY400750
Transient overexpression lysate of growth associated protein 43 (GAP43), transcript variant 2 (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC203175 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_002045, NP_002036 |
| Synonyms | B-50; GAP-43; PP46 |
| Predicted MW | 24.8 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_002036 |
| Locus ID | 2596 |
| Cytogenetics | 3q13.31 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "GAP43" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303175 | GAP43 MS Standard C13 and N15-labeled recombinant protein (NP_002036) | 10 ug |
$3,360.00
|
|
| LC400750 | Lyophilized GAP43 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP303175 | Recombinant protein of human growth associated protein 43 (GAP43), transcript variant 2, 20 µg | 20 ug |
$867.00
|
|
Citations
Intracellular cavity of sensor domain controls allosteric gating of TRPA1 channel
Zimova, Sinica, Kadkova et al
Sci Signal (2018) 11 (514) DOI: 10.1126/scisignal.aan8621
Zimova, Sinica, Kadkova et al
Sci Signal (2018) 11 (514) DOI: 10.1126/scisignal.aan8621
AI Summary: OriGene’s pCMV6-XL4 and pCMV6-XL5 plasmids encoding wild-type or mutant human TRPA1, GAP43, and MARCKS were used for transient transfection in HEK293T cells to investigate the molecular mechanisms underlying TRPA1 channel gating.
Snippet: … The magnet-assisted transfection (IBA GmbH) technique was used to transiently cotransfect the cells in a 15.6-mm well on a 24-well plate with 200 ng of plasmid encoding green fluorescent protein (TaKaRa), 300 ng of plasmid encoding wild-type or mutant human TRPA1 (pCMV6-XL4 vector, OriGene), and, for particular experiments, 200 ng of plasmid of wild-type or mutant GAP43 (pCMV6-XL5 vector, OriGene) or 100 ng of plasmid encoding MARCKS (pCMV6-XL5 vector, OriGene)
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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