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EIF4A2 (NM_001967) Human Over-expression Lysate

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SKU
LY400722
Transient overexpression lysate of eukaryotic translation initiation factor 4A2 (EIF4A2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205623
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001967, NP_001958
Synonyms BM-010; DDX2B; eIF-4A-II; EIF4A; eIF4A-II; EIF4F
Predicted MW 46.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001958
Locus ID 1974
Cytogenetics 3q27.3
Protein Families WB
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Other "EIF4A2" proteins (3)
SKU Description Size Price
PH305623 EIF4A2 MS Standard C13 and N15-labeled recombinant protein (NP_001958) 10 ug
$3,360.00
LC400722 Lyophilized EIF4A2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP305623 Recombinant protein of human eukaryotic translation initiation factor 4A, isoform 2 (EIF4A2), 20 µg 20 ug
$737.00

file-alt Citations

Functional mimicry revealed by the crystal structure of an eIF4A:RNA complex bound to the interfacial inhibitor, desmethyl pateamine A
Naineni, Liang, Hull et al
Cell Chem Biol (2021)
Cited by 45
AI Summary: OriGene's TurboFectin 8.0 was used for transfecting eIF4A2-eHAP1 cells, facilitating the cellular experiments that supported the structural and functional analysis in this study.
Snippet: ’. Following transfection intoEIF4A2-eHAP1 cells using TurboFectin 8.0 (Origene), cells were given 48 h to recover before being sorted into 96 well plates and undergoingselection with 50 nM CR-1-31-B
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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