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E2F4 (NM_001950) Human Over-expression Lysate

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SKU
LY400717
Transient overexpression lysate of E2F transcription factor 4, p107/p130-binding (E2F4) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207699
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001950, NP_001941
Synonyms E2F-4
Predicted MW 44 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001941
Locus ID 1874
Cytogenetics 16q22.1
Protein Families WB
Protein Pathways Cell cycle, TGF-beta signaling pathway
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Other "E2F4" proteins (1)
SKU Description Size Price
LC400717 Lyophilized E2F4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

CDC20B is required for deuterosome-mediated centriole production in multiciliated cells
Revinski, Zaragosi, Boutin et al
Nat Commun (2018) 9 (1), 4668 DOI: 10.1038/s41467-018-06768-z
Cited by 87
AI Summary: OriGene products contributed to this study by providing the CDC20B Myc-DDK tagged expression plasmid (Cat# RC223434), which was used for CDC20B overexpression experiments to investigate its role in centriole production in multiciliated cells.
FigureFigureFigure
Showing 1-1 of 1 papers.
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