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Caveolin 1 (CAV1) (NM_001753) Human Over-expression Lysate

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SKU
LY400664
Transient overexpression lysate of caveolin 1, caveolae protein, 22kDa (CAV1) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210274
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001753, NP_001744
Synonyms BSCL3; CGL3; LCCNS; MSTP085; PPH3; VIP21
Predicted MW 20.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001744
Locus ID 857
Cytogenetics 7q31.2
Protein Families WB
Protein Pathways Focal adhesion, Viral myocarditis
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Other "Caveolin 1" proteins (3)
SKU Description Size Price
LC400664 Lyophilized CAV1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC432589 Lyophilized CAV1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY432589 Transient overexpression lysate of caveolin 1, caveolae protein, 22kDa (CAV1), transcript variant 2 (as WB positive control) 100 ug
$436.00

file-alt Citations

Deficiency of cold-inducible RNA-binding protein exacerbated monocrotaline-induced pulmonary artery hypertension through Caveolin1 and CAVIN1
Liu, Ke, Wang et al
J Cell Mol Med (2021)
Cited by 17
AI Summary: OriGene's pCMV6-Myc-DDK expression vectors were used to clone and overexpress Cirp, Cav1, and Cavin1 cDNAs in rat pulmonary artery endothelial cells, enabling functional studies of these genes in pulmonary artery hypertension.
Snippet: Page 3. | 3 LIU et aL. For overexpression, Cirp, Cav1 and Cavin1 cDNAs were cloned into pCMV6 tagged with Myc and DDK (Origene), and verified by Sanger sequencing. The constructs were transfected into rPAECs with Lipofectamine 3000 (Invitrogen);- Page 3. | 3 LIU et aL. For overexpression, Cirp, Cav1 and Cavin1 cDNAs were cloned into pCMV6 tagged with Myc and DDK (Origene), and verified by Sanger sequencing. …
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum
Shaner, Lambert, Chammas et al
Nat. Methods (2013) 10 (5), 407-9 DOI: 10.1038/nmeth.2413
Cited by 1516
AI Summary: OriGene contributed mouse β-catenin cDNA (product: NM_001165902.1) used for cloning and expression experiments in the study.
Snippet: routed by a beam-splitter through the 530/43 nm (acceptor emission) and the 480/40 nm (donor emission) band-pass emission ... NM_001101.3);β-catenin (20 aa), coding sequence cloned between XhoI and BamHI (mouse β-catenin;cDNA source: Origene;NM_001165902.1
An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore
Subach, Cranfill, Davidson et al
PLoS ONE (2011) 6 (12), e28674 DOI: 10.1371/journal.pone.0028674
Cited by 402
AI Summary: OriGene contributed human VE-cadherin cDNA (catalog reference: NM_003461) used in this study to create protein fusions for evaluating the properties of the enhanced blue fluorescent protein mTagBFP2.
Snippet: cDNA source: Origene, Rockville, MD;NM_003461);vascular epithelial cadherin (10), AgeI and NotI (human VE cadherin cDNA source: Origene, Rockville, MD ... Photostability is time for photobleaching from an initial emission rate of 1,000 photons/s down to 500 photons/s [13].
FigureFigureFigure
Showing 1-3 of 3 papers.
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