DAB2 (NM_001343) Human Over-expression Lysate
SKU
LY400534
Transient overexpression lysate of disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila) (DAB2) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC200481 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001343, NP_001334 |
| Synonyms | DOC-2; DOC2 |
| Predicted MW | 82.3 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001334 |
| Locus ID | 1601 |
| Cytogenetics | 5p13.1 |
| Protein Families | WB |
| Protein Pathways | Endocytosis |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "DAB2" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH300481 | DAB2 MS Standard C13 and N15-labeled recombinant protein (NP_001334) | 10 ug |
$3,360.00
|
|
| LC400534 | Lyophilized DAB2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP300481 | Recombinant protein of human disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila) (DAB2), 20 µg | 20 ug |
$867.00
|
|
Citations
The histone demethylase Kdm3a is essential to progression through differentiation
Herzog, Josseaux, Dedeurwaerder et al
Nucleic Acids Res. (2012) 40 (15), 7219-32 DOI: 10.1093/nar/gks399
Herzog, Josseaux, Dedeurwaerder et al
Nucleic Acids Res. (2012) 40 (15), 7219-32 DOI: 10.1093/nar/gks399
Cited by 33
AI Summary: OriGene's Dab2 cDNA clone (MC200502) was used to generate expression constructs for functional studies investigating the role of Dab2 in cell differentiation in this paper.
Snippet: Dab2 cDNA was subcloned from a commercially available clone (Origene, MC200502) into pcDNA3.1-HA (CMV promoter ... from independent differentiation experiments for gene expression, the very high reproducibility of the microarray data further suggests good robustness for
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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