CLIC1 (NM_001288) Human Over-expression Lysate
SKU
LY400515
Transient overexpression lysate of chloride intracellular channel 1 (CLIC1) (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC218042 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001288, NP_001279 |
| Synonyms | CL1C1; CLCNL1; G6; NCC27 |
| Predicted MW | 26.7 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001279 |
| Locus ID | 1192 |
| Cytogenetics | 6p21.33 |
| Protein Families | WB |
Reviews
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Resources
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Other "CLIC1" proteins (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH318042 | CLIC1 MS Standard C13 and N15-labeled recombinant protein (NP_001279) | 10 ug |
$3,360.00
|
|
| LC400515 | Lyophilized CLIC1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP318042 | Recombinant protein of human chloride intracellular channel 1 (CLIC1), 20 µg | 20 ug |
$867.00
|
|
| TP720956 | Purified recombinant protein of Human chloride intracellular channel 1 (CLIC1) | 10 ug |
$260.00
|
|
Citations
The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility
Stakaitytė, Nwogu, Lippiat et al
J. Biol. Chem. (2018)
Stakaitytė, Nwogu, Lippiat et al
J. Biol. Chem. (2018)
AI Summary: OriGene contributed to this study by providing the EGFP-CLIC1 expression construct and formalin-fixed, paraffin-embedded (FFPE) sections from primary MCC tumors, which were used to investigate the role of CLIC1 and CLIC4 chloride channels in virus-mediated cell motility.
Snippet: EGFP-CLIC1 expression construct was purchased from Origene Multicolour immunohistochemistry - Formalin-fixed, paraffin-embedded (FFPE) sections from primary MCC tumours were procured from Origene and analysed as previously described (41)
Showing 1-1 of 1 papers.
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