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Bak (BAK1) (NM_001188) Human Over-expression Lysate

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SKU
LY400476
Transient overexpression lysate of BCL2-antagonist/killer 1 (BAK1) (as WB positive control)
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201216
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001188, NP_001179
Synonyms BAK; BAK-LIKE; BCL2L7; CDN1
Predicted MW 23.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001179
Locus ID 578
Cytogenetics 6p21.31
Protein Families WB
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Documents
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Other "Bak" proteins (1)
SKU Description Size Price
LC400476 Lyophilized BAK1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

MicroRNA-29c Correlates with Neuroprotection Induced by FNS by Targeting Both Birc2 and Bak1 in Rat Brain after Stroke
Huang, Li, Pang et al
CNS Neurosci Ther (2015)
Cited by 43
AI Summary: In this study, the OriGene Bak1 antibody (TA302647) was used to detect Bak1 protein levels by Western blot, helping to assess how microRNA-29c regulates Bak1 expression in the neuroprotective effects of fastigial nucleus stimulation after stroke.
Snippet: Abcam, Cambridge, UK) and GAPDH (1:5000;AG019, Beyotime), and then washed and incubated with secondary 1:10,000 anti-rabbit antibody (042-06-18-06;KPL, Washington, USA) at room temperature for 2 h. There also are Bak1 (1:500, TA302647;Origene, MD ... Cite SaveSaving
WB
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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