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RUNX3 (NM_001031680) Human Over-expression Lysate

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SKU
LY400418
Transient overexpression lysate of runt-related transcription factor 3 (RUNX3), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203597
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001031680, NP_001026850
Synonyms AML2; CBFA3; PEBP2aC
Predicted MW 45.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001026850
Locus ID 864
Cytogenetics 1p36.11
Protein Families WB
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Other "RUNX3" proteins (2)
SKU Description Size Price
LC400418 Lyophilized RUNX3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP760558 Purified recombinant protein of Human runt-related transcription factor 3 (RUNX3), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Inflammation-driven colon neoplasmatogenesis in uPA-deficient mice is associated with an increased expression of Runx transcriptional regulators
Afaloniati, Karagiannis, Hardas et al
Exp Cell Res (2017) 361 (2), 257-264 DOI: 10.1016/j.yexcr.2017.10.025
Cited by 8
AI Summary: OriGene products, specifically the anti-Runx1 (Cat# TA324352), anti-Runx2 (Cat# TA327322), and anti-Runx3 (Cat# TA327321) antibodies, were used in this study to detect and analyze the expression of Runx transcription factors in mouse colon tissues, thereby contributing to the identification of increased Runx1/2/3 expression associated with inflammation-driven colon cancer in uPA-deficient mice.
Showing 1-1 of 1 papers.
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