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P4HB (NM_000918) Human Over-expression Lysate

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SKU
LY400335
Transient overexpression lysate of prolyl 4-hydroxylase, beta polypeptide (P4HB) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202275
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000918, NP_000909
Synonyms CLCRP1; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB
Predicted MW 57.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000909
Locus ID 5034
Cytogenetics 17q25.3
Protein Families WB
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Other "P4HB" proteins (4)
SKU Description Size Price
PH302275 P4HB MS Standard C13 and N15-labeled recombinant protein (NP_000909) 10 ug
$3,360.00
LC400335 Lyophilized P4HB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302275 Recombinant protein of human prolyl 4-hydroxylase, beta polypeptide (P4HB), 20 µg 20 ug
$737.00
TP720913 Purified recombinant protein of Human prolyl 4-hydroxylase, beta polypeptide (P4HB) 10 ug
$260.00

file-alt Citations

Endoplasmic reticulum chaperone prolyl 4-hydroxylase, beta polypeptide (P4HB) promotes malignant phenotypes in glioma via MAPK signaling
Sun, Kiang, Ho et al
Oncotarget (2017) 8 (42), 71911-71923 DOI: 10.18632/oncotarget.18026
Snippet: P4HB human cDNA clone was obtained from OriGene Technologies (Rockville, MD, USA). To establish P4HB over-expressed cells, full-length P4HB cDNA flanked with EcoRI and Not I restriction sites was ligated to pcDNA3.1/V5-His C expression plasmid (Invitrogen;Life Technologies)
FigureFigureFigure
Inhibition of prolyl 4-hydroxylase, beta polypeptide (P4HB) attenuates temozolomide resistance in malignant glioma via the endoplasmic reticulum stress response (ERSR) pathways
Sun, Lee, Ho et al
Neuro-oncology (2013) 15 (5), 562-77 DOI: 10.1093/neuonc/not005
Cited by 93
AI Summary: OriGene’s pGFP-V-RS plasmid encoding P4HB-specific shRNA was used to knock down P4HB expression in glioma cells, enabling the study to demonstrate that reducing P4HB sensitizes cells to temozolomide and helps overcome drug resistance.
Snippet: In addition, D54-R and U87-R cells were manipulated with P4HB-specific shRNA (shP4HB) encoding pGFP-V-RS plasmid (OriGene Technologies, Inc., Rockville, MD). Green fluorescent protein (GFP) was used to monitor transfection efficiency.
Showing 1-2 of 2 papers.
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