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FOLR2 (NM_000803) Human Over-expression Lysate

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SKU
LY400280
Transient overexpression lysate of folate receptor 2 (fetal) (FOLR2), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212993
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000803, NP_000794
Synonyms BETA-HFR; FBP; FBP/PL-1; FOLR1; FR-BETA; FR-P3; FRbeta
Predicted MW 29.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000794
Locus ID 2350
Cytogenetics 11q13.4
Protein Families WB
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Other "FOLR2" proteins (8)
SKU Description Size Price
LC400280 Lyophilized FOLR2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426430 Lyophilized FOLR2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426431 Lyophilized FOLR2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426432 Lyophilized FOLR2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY426430 Transient overexpression lysate of folate receptor 2 (fetal) (FOLR2), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY426431 Transient overexpression lysate of folate receptor 2 (fetal) (FOLR2), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY426432 Transient overexpression lysate of folate receptor 2 (fetal) (FOLR2), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP762242 Purified recombinant protein of Human folate receptor 2 (fetal) (FOLR2), transcript variant 1, Ser20-Arg221, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Growing Mouse Oocytes Transiently Activate Folate Transport via Folate Receptors As They Approach Full Size
Meredith, MacNeil, Trasler et al
Biol. Reprod. (2016)
Cited by 24
AI Summary: OriGene’s human FOLR2 overexpression lysate (LY400280) was used in deglycosylation experiments to confirm enzyme activity in the study of folate receptor function during mouse oocyte growth.
Snippet: Deglycosylation of RNAse B (5 µg;P7817S, New England Biolabs) and of human FOLR2 overexpressed in HEK293T cells (LY400280, OriGene, Rockville, MD) was used to confirm activity. Negative control samples had water rather than PNGase F solution added. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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