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CYP11B2 (NM_000498) Human Over-expression Lysate

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SKU
LY400169
Transient overexpression lysate of cytochrome P450, family 11, subfamily B, polypeptide 2 (CYP11B2), nuclear gene encoding mitochondrial protein (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215476
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000498, NP_000489
Synonyms ALDOS; CPN2; CYP11B; CYP11BL; CYPXIB2; P-450C18; P450aldo; P450C18
Predicted MW 57.56 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000489
Locus ID 1585
Cytogenetics 8q24.3
Protein Families WB
Protein Pathways C21-Steroid hormone metabolism, Metabolic pathways
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Other "CYP11B2 (CYP11B2)" proteins (1)
SKU Description Size Price
LC400169 Lyophilized CYP11B2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Analysis of novel heterozygous mutations in the CYP11B2 gene causing congenital aldosterone synthase deficiency and literature review
Miao, Yu, Lu et al
Steroids (2019) 150, 108448 DOI: 10.1016/j.steroids.2019.108448
AI Summary: OriGene's pcDNA3 plasmid and full-length human CYP11B2 cDNA (SC102224) were used to generate mutant constructs for functional analysis of CYP11B2 variants in this study.
Snippet: C. 2.3. CYP11B2 expression and enzymatic activity. 2.3.1. Site-directed mutagenesis in pcDNA3-CYP11B2. pcDNA3 plasmid and full-length human CYP11B2 cDNA were obtained from OriGene (SC102224, Rockville, MD). QuikChange
A Novel KCNJ5-insT149 Somatic Mutation Close to, but Outside, the Selectivity Filter Causes Resistant Hypertension by Loss of Selectivity for Potassium
Kuppusamy, Caroccia, Stindl et al
J. Clin. Endocrinol. Metab. (2014)
Cited by 57
AI Summary: OriGene products contributed to this study by providing human full-length cDNA plasmids for KCNJ3 (SC118769) and KCNJ5 (SC119590), which were used to transfect adrenocortical cells for functional analysis of the novel KCNJ5-insT149 mutation.
Snippet: Plasmids For studies of CYP11B1 and CYP11B2 expression and aldo- sterone production, adrenocortical HAC15 were transfected with human full-length cDNA of KCNJ3 (SC118769;Origene) and of KCNJ5 (SC119590), in which the KCNJ5 mutations (T158A and ... Cite SaveSaving
Showing 1-2 of 2 papers.
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