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Integrin alpha 6 (ITGA6) (NM_000210) Human Over-expression Lysate

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SKU
LY400075
Transient overexpression lysate of integrin, alpha 6 (ITGA6), transcript variant 2 (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221990
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000210, NP_000201
Synonyms CD49f; ITGA6B; VLA-6
Predicted MW 119.48 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000201
Locus ID 3655
Cytogenetics 2q31.1
Protein Families WB
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer
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Other "Integrin alpha 6" proteins (3)
SKU Description Size Price
PH321990 ITGA6 MS Standard C13 and N15-labeled recombinant protein (NP_000201) 10 ug
$3,360.00
LC400075 Lyophilized ITGA6 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP321990 Recombinant protein of human integrin, alpha 6 (ITGA6), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

CD49f Can Act as a Biomarker for Local or Distant Recurrence in Breast Cancer
Ye, Zhong, Qiu et al
J Breast Cancer (2017) 20 (2), 142-149 DOI: 10.4048/jbc.2017.20.2.142
AI Summary: OriGene’s anti-CD49f antibody (TA506627) was used for immunohistochemical staining to detect CD49f expression in breast cancer tissue samples, enabling the study to assess its value as a biomarker for disease recurrence.
Snippet: Immunohistochemical staining. The procedure for IHC staining was performed as previously reported [2]. Antibody detection of CD49f (TA506627, 1:150;OriGene, Rockville, USA) was performed using an automated staining machine according to the manufacturer's ... All 11 versions Cite SaveSaving
FigureFigureFigure
IHC
Showing 1-1 of 1 papers.
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