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Protein Kinase A regulatory subunit I alpha (PRKAR1A) (NM_212471) Human Over-expression Lysate

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SKU
LC431006
Lyophilized PRKAR1A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Other products for "Protein Kinase A regulatory subunit I alpha or NM_212471"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210623
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_212471, NP_997636
Synonyms ACRDYS1; ADOHR; CAR; CNC; CNC1; PKR1; PPNAD1; PRKAR1; TSE1
Predicted MW 43 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_997636
Locus ID 5573
Cytogenetics 17q24.2
Protein Families WB
Protein Pathways Apoptosis, Insulin signaling pathway
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Other "Protein Kinase A regulatory subunit I alpha" proteins (13)
SKU Description Size Price
PH303828 PRKAR1A MS Standard C13 and N15-labeled recombinant protein (NP_002725) 10 ug
$3,360.00
PH310623 PRKAR1A MS Standard C13 and N15-labeled recombinant protein (NP_997636) 10 ug
$3,360.00
PH312810 PRKAR1A MS Standard C13 and N15-labeled recombinant protein (NP_997637) 10 ug
$3,360.00
LC400963 Lyophilized PRKAR1A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC403944 Lyophilized PRKAR1A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC403945 Lyophilized PRKAR1A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400963 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY403944 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY403945 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY431006 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP303828 Recombinant protein of human protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 1, 20 µg 20 ug
$737.00
TP310623 Recombinant protein of human protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 2, 20 µg 20 ug
$737.00
TP312810 Recombinant protein of human protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) (PRKAR1A), transcript variant 3, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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