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TCN2 (NM_000355) Human Over-expression Lysate

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SKU
LC424773
Lyophilized TCN2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200682
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000355, NP_000346
Synonyms D22S676; D22S750; II; TC; TC-2; TC2; TC II; TCII
Predicted MW 47.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000346
Locus ID 6948
Cytogenetics 22q12.2
Protein Families WB
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Other "TCN2" proteins (3)
SKU Description Size Price
LC433003 Lyophilized TCN2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY424773 Transient overexpression lysate of transcobalamin II; macrocytic anemia (TCN2) (as WB positive control) 100 ug
$436.00
LY433003 Transient overexpression lysate of transcobalamin II (TCN2), transcript variant 2 (as WB positive control) 100 ug
$436.00

file-alt Citations

Two-step activation prodrugs: transplatin mediated binding of chemotherapeutic agents to vitamin B12
Tran, Furger, Alberto
Org. Biomol. Chem. (2013) 11 (19), 3247-54 DOI: 10.1039/c3ob40093j
Cited by 19
AI Summary: In this study, OriGene’s anti-cytarabine antibody (TA326106) was used to detect and quantify cytarabine released from the prodrug, thereby helping to evaluate the effectiveness of the two-step activation and drug release process.
Snippet: competes with deoxycytidine triphosphate in DNA synthesis. However, Cyt represents a challenge for tumor targeting due to its short plasma half-life, low stability and limited bioavailability. Furthermore, low membrane permeability and the ease to be inactivated by
Showing 1-1 of 1 papers.
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