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SS18 (NM_001007559) Human Over-expression Lysate

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SKU
LC423511
Lyophilized SS18 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219498
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001007559, NP_001007560
Synonyms SSXT; SYT
Predicted MW 45.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001007560
Locus ID 6760
Cytogenetics 18q11.2
Protein Families WB
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Other "SS18" proteins (4)
SKU Description Size Price
LC417168 Lyophilized SS18 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417168 Transient overexpression lysate of synovial sarcoma translocation, chromosome 18 (SS18), transcript variant 2 (as WB positive control) 100 ug
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LY423511 Transient overexpression lysate of synovial sarcoma translocation, chromosome 18 (SS18), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP762305 Purified recombinant protein of Human synovial sarcoma translocation, chromosome 18 (SS18), transcript variant 1, Gly227-Pro255, with N-terminal His-ABP tag, expressed in E.coli, 50ug 50 ug
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file-alt Citations

The clinical implication of SS18-SSX fusion gene in synovial sarcoma
Ren, Lu, Guo et al
Br. J. Cancer (2013)
Cited by 40
AI Summary: OriGene products SS18-SSX1 (Cat. No: RG219498) and SS18-SSX2 (Cat. No: RG215192) recombinant plasmids were used to transfect cell lines, enabling the study of how each SS18-SSX fusion gene variant affects cell proliferation and invasion in synovial sarcoma.
Snippet: GFP separately (purchased from OriGene Technologies, Inc., Cat. No: RG219498 and RG215192, respectively). 293T cells were transfected with the recombinant plasmids and vector alone (mock transfectants) using Lipofectamine 2000 reagent (Life Technologies, Carlsbad
Showing 1-1 of 1 papers.
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