AKR1C1 (NM_001353) Human Over-expression Lysate
SKU
LC419968
Lyophilized AKR1C1 HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC207278 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001353, NP_001344 |
| Synonyms | 2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1; DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB |
| Predicted MW | 36.6 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_001344 |
| Locus ID | 1645 |
| Cytogenetics | 10p15.1 |
| Protein Families | WB |
| Protein Pathways | Metabolism of xenobiotics by cytochrome P450 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "AKR1C1" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LY419968 | Transient overexpression lysate of aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) (AKR1C1) (as WB positive control) | 100 ug |
$436.00
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Citations
The roles of AKR1C1 and AKR1C2 in ethyl-3,4-dihydroxybenzoate induced esophageal squamous cell carcinoma cell death
Li, Hou, Zhou et al
Oncotarget (2016) 7 (16), 21542-55 DOI: 10.18632/oncotarget.7775
Li, Hou, Zhou et al
Oncotarget (2016) 7 (16), 21542-55 DOI: 10.18632/oncotarget.7775
Cited by 21
AI Summary: OriGene’s full-length AKR1C1 DNA (NM_001353) was used to clone and produce recombinant AKR1C1 protein, enabling functional studies of its role in ethyl-3,4-dihydroxybenzoate-induced esophageal cancer cell death.
Snippet: Cloning, production, and purification of recombinant AKR1C1 proteins DNA encoding full-length AKR1C1 (accession number NM_001353) was obtained from OriGene and cloned into pEX-N-His. Plasmids were transformed into Escherichia coli
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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