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CPT1A (NM_001876) Human Over-expression Lysate

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SKU
LC419675
Lyophilized CPT1A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221740
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001876, NP_001867
Synonyms CPT1; CPT1-L; L-CPT1
Predicted MW 88.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001867
Locus ID 1374
Cytogenetics 11q13.3
Protein Families WB
Protein Pathways Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway
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Other "CPT1A" proteins (5)
SKU Description Size Price
PH321740 CPT1A MS Standard C13 and N15-labeled recombinant protein (NP_001867) 10 ug
$3,360.00
LC422204 Lyophilized CPT1A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY419675 Transient overexpression lysate of carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1 (as WB positive control) 100 ug
$665.00
LY422204 Transient overexpression lysate of carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2 (as WB positive control) 100 ug
$436.00
TP321740 Recombinant protein of human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

IL-9/STAT3/fatty acid oxidation-mediated lipid peroxidation contributes to Tc9 cell longevity and enhanced antitumor activity
Xiao, Ma, Ye et al
J Clin Invest (2022) 132 (7) DOI: 10.1172/JCI153247
AI Summary: OriGene’s Cpt1a cDNA was used to overexpress Cpt1a in Tc9 cells, enabling the authors to investigate the role of fatty acid oxidation in Tc9 cell longevity and antitumor activity.
Snippet: …For Cpt1a overexpression, cDNA encoding Cpt1a was purchased from OriGene and subcloned into the MigR1 retroviral vector (64). … Viral supernatant was harvested 48 hours after transfection and;- For Cpt1a overexpress, complementary DNAs (cDNAs) encoding Cpt1a were purchased from OriGene and subcloned into the MigR1 retroviral vector (64). …
Showing 1-1 of 1 papers.
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