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MAFG (NM_002359) Human Over-expression Lysate

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SKU
LC419371
Lyophilized MAFG HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221486
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002359, NP_002350
Synonyms hMAF
Predicted MW 17.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002350
Locus ID 4097
Cytogenetics 17q25.3
Protein Families WB
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Other "MAFG" proteins (5)
SKU Description Size Price
PH321486 MAFG MS Standard C13 and N15-labeled recombinant protein (NP_002350) 10 ug
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LC409976 Lyophilized MAFG HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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file-alt Citations

DNA Methylation of miR-7 is a Mechanism Involved in Platinum Response through MAFG Overexpression in Cancer Cells
Vera, Jimenez, Pernia et al
Theranostics (2017) 7 (17), 4118-4134 DOI: 10.7150/thno.20112
Cited by 64
AI Summary: In this study, OriGene’s MAFG expression plasmid (RC208447) and MAFG shRNA plasmids (TG311804, TG311805) were used for functional assays to investigate the regulatory relationship between miR-7 and MAFG, thereby elucidating their roles in platinum resistance in cancer cells.
FigureFigureFigure
Showing 1-1 of 1 papers.
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