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PRKAR2B (NM_002736) Human Over-expression Lysate

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SKU
LC419138
Lyophilized PRKAR2B HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209900
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002736, NP_002727
Synonyms PRKAR2; RII-BETA
Predicted MW 46.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002727
Locus ID 5577
Cytogenetics 7q22.3
Protein Families WB
Protein Pathways Apoptosis, Insulin signaling pathway
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Other "PRKAR2B" proteins (3)
SKU Description Size Price
PH309900 PRKAR2B MS Standard C13 and N15-labeled recombinant protein (NP_002727) 10 ug
$3,360.00
LY419138 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type II, beta (PRKAR2B) (as WB positive control) 100 ug
$436.00
TP309900 Recombinant protein of human protein kinase, cAMP-dependent, regulatory, type II, beta (PRKAR2B), 20 µg 20 ug
$867.00

file-alt Citations

Hypoxia and the hypoxia inducible factor 1α activate protein kinase A by repressing RII beta subunit transcription
Lucia, Wu, Garcia et al
Oncogene (2020) 39 (16), 3367-3380 DOI: 10.1038/s41388-020-1223-6
Cited by 29
AI Summary: OriGene’s PRKAR2B expression plasmid (SKU SC125501) was used in this study to investigate the role of the PKA regulatory subunit in hypoxia-induced PKA activation, helping to elucidate the mechanism by which HIF-1α represses PRKAR2B transcription.
Snippet: …sc-45919) and rat Creb1 (Santa Cruz Cat. sc72030) and nonspecific scramble control (Santa Cruz Cat. sc37007). The following expression plasmids were used: M7 pdn-PKA-GFP (Randall Moon;Addgene plasmid # 16716), RSV CREB (Marc Montminy;Addgene plasmid # 22394), RSV CREB-M1 (Marc Montminy;Addgene plasmid # 22395), PRKAR2B (Origene, SKU SC125501), and pCMV3xFLAG-HIF-1α (gift of Eduardo Arzt (Buenos Aires Argentina)). …
Showing 1-1 of 1 papers.
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