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SLC6A12 (NM_003044) Human Over-expression Lysate

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SKU
LC418940
Lyophilized SLC6A12 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222855
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003044, NP_003035
Synonyms BGT-1; BGT1; GAT2
Predicted MW 69.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003035
Locus ID 6539
Cytogenetics 12p13.33
Protein Families WB
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Other "SLC6A12" proteins (5)
SKU Description Size Price
LC426578 Lyophilized SLC6A12 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC426579 Lyophilized SLC6A12 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY418940 Transient overexpression lysate of solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 1 (as WB positive control) 100 ug
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LY426578 Transient overexpression lysate of solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 2 (as WB positive control) 100 ug
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LY426579 Transient overexpression lysate of solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 3 (as WB positive control) 100 ug
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file-alt Citations

Aberrant Hypomethylation of Solute Carrier Family 6 Member 12 Promoter Induces Metastasis of Ovarian Cancer
Sung, Yang, Park et al
Yonsei Med. J. (2017) 58 (1), 27-34 DOI: 10.3349/ymj.2017.58.1.27
AI Summary: OriGene’s pCMV6-SLC6A12 plasmid was used for transient overexpression of SLC6A12 in SK-OV-3 ovarian cancer cells, enabling the study of its role in cancer metastasis.
Snippet: Transient transfection. To establish a transient expression system, SK-OV-3 cellswere transfected with pCMV6-SLC6A12 (Origene, Rockville, MD, USA) or pEGFP-N3 (Clontech,Mountain View, CA, USA) plasmids using Lipofectamine 2000 (Invitrogen). ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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