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SOX11 (NM_003108) Human Over-expression Lysate

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SKU
LC418895
Lyophilized SOX11 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC220681
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003108, NP_003099
Synonyms CSS9; MRD27
Predicted MW 46.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003099
Locus ID 6664
Cytogenetics 2p25.2
Protein Families WB
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Other "SOX11" proteins (2)
SKU Description Size Price
LY418895 Transient overexpression lysate of SRY (sex determining region Y)-box 11 (SOX11) (as WB positive control) 100 ug
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TP762361 Purified recombinant protein of Human SRY (sex determining region Y)-box 11 (SOX11), Ala157-Glu224-GGGGS-Glu242-Ala334, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization
Chiang, Wu, Lin et al
Sci Adv (2021) 7 (7) DOI: 10.1126/sciadv.abc6093
Cited by 45
AI Summary: OriGene products contributed to this study by providing the Hes1 and Sox11 cDNAs, which were used to investigate the molecular mechanisms underlying Usp11's role in cortical neurogenesis and neuronal migration.
Snippet: and pBybe-myc. Usp11 C275/283S (CS) and R241Q mutants and human USP11 R241Q mutant were generated by Pfu site-directed mutagenesis (Thermo Fisher Scientific). Hes1 and Sox11 cDNAs were purchased from Origene and subcloned to pRK5-Flag and/or pUS2. To clone Usp11 promoter, a DNA fragment corresponding to nucleotides from −1667 to +343 of the mouse Usp11 gene was amplified from the genomic DNA of
FigureFigureFigure
Embryonic mammary signature subsets are activated in Brca1-/- and basal-like breast cancers
Zvelebil, Oliemuller, Gao et al
Breast Cancer Res. (2013) 15 (2), R25 DOI: 10.1186/bcr3403
Cited by 66
AI Summary: OriGene's pCMV6-AC-GFP plasmid containing the SOX11-GFP fusion protein (catalog number RG220681) was used to transfect BT549 cells, enabling the study of SOX11's role in breast cancer cell proliferation and survival in this research.
Snippet: 1x106 BT549 cells were transfected with 3 µg of either pCMV6-AC-GFP plasmid containing the sequence for a fusion protein between SOX11 and GFP (RG220681, Origene) or a ... hours with PrestoBlue Cell Viability Reagent (Life Technologies). The absorbance obtained
Showing 1-2 of 2 papers.
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