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TLR2 (NM_003264) Human Over-expression Lysate

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SKU
LC418800
Lyophilized TLR2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207597
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003264, NP_003255
Synonyms CD282; TIL4
Predicted MW 89.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003255
Locus ID 7097
Cytogenetics 4q31.3
Protein Families WB
Protein Pathways Toll-like receptor signaling pathway
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Other "TLR2" proteins (3)
SKU Description Size Price
PH307597 TLR2 MS Standard C13 and N15-labeled recombinant protein (NP_003255) 10 ug
$3,360.00
LY418800 Transient overexpression lysate of toll-like receptor 2 (TLR2) (as WB positive control) 100 ug
$436.00
TP307597 Recombinant protein of human toll-like receptor 2 (TLR2), 20 µg 20 ug
$867.00

file-alt Citations

Selective disruption of TLR2/MyD88 interaction inhibits inflammation and attenuates Alzheimer's pathology
Rangasamy, Jana, Roy et al
J. Clin. Invest. (2018)
AI Summary: OriGene’s pLenti-cmyc-DDK/tlr2 full-length construct was used to generate a C-terminal TLR2 (cTLR2) expression vector, enabling the study of TLR2/MyD88 interactions and their role in inflammation and Alzheimer's pathology.
Snippet: normalized to loading control (β-actin). Preparation of C-terminal TLR2 (cTLR2) TLR2 full-length construct (pLenti-cmyc-DDK/ tlr2) was purchased from Origene. The cTLR2 (640-784 amino acids) tagged with c-myc was cloned in lenti vector using TOPO TA cloning
Showing 1-1 of 1 papers.
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