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MSK2 / RSK-B (RPS6KA4) (NM_003942) Human Over-expression Lysate

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SKU
LC418335
Lyophilized RPS6KA4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212570
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003942, NP_003933
Synonyms MSK2; RSK-B; S6K-alpha-4
Predicted MW 85.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003933
Locus ID 8986
Cytogenetics 11q13.1
Protein Families WB
Protein Pathways MAPK signaling pathway, Neurotrophin signaling pathway
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Other "MSK2 / RSK-B" proteins (5)
SKU Description Size Price
PH312570 RPS6KA4 MS Standard C13 and N15-labeled recombinant protein (NP_003933) 10 ug
$3,360.00
LC423559 Lyophilized RPS6KA4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY418335 Transient overexpression lysate of ribosomal protein S6 kinase, 90kDa, polypeptide 4 (RPS6KA4), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY423559 Transient overexpression lysate of ribosomal protein S6 kinase, 90kDa, polypeptide 4 (RPS6KA4), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP312570 Recombinant protein of human ribosomal protein S6 kinase, 90kDa, polypeptide 4 (RPS6KA4), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy
Badal, Wang, Long et al
Nat Commun (2016) 7, 12076 DOI: 10.1038/ncomms12076
Cited by 69
AI Summary: OriGene’s Msk2 shRNA (mouse Rps6ka4, 29mer shRNA constructs in an untagged retroviral vector) and scramble control shRNA were used to achieve Msk2 knockdown in mice, enabling the study of miR-93’s role in chromatin remodeling during diabetic nephropathy.
Snippet: c knockdown of Msk2 was achieved by delivering Msk2 shRNA (mouse Rps6ka4, 29mer shRNA constructs in an untagged retroviral vector) or a scramble control shRNA construct purchased from OriGene (Rockville, MD, USA) with the kidney in vivo transfection reagent (KIDNEY
FigureFigureFigure
Showing 1-1 of 1 papers.
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