CCS (NM_005125) Human Over-expression Lysate
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC211311 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_005125, NP_005116 |
| Predicted MW | 28.9 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_005116 |
| Locus ID | 9973 |
| Cytogenetics | 11q13.2 |
| Protein Families | WB |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS) |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "CCS" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LY417482 | Transient overexpression lysate of copper chaperone for superoxide dismutase (CCS) (as WB positive control) | 100 ug |
$436.00
|
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Citations
Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1
Maryon, Molloy, Kaplan
Am. J. Physiol., Cell Physiol. (2013) 304 (8), C768-79 DOI: 10.1152/ajpcell.00417.2012
Maryon, Molloy, Kaplan
Am. J. Physiol., Cell Physiol. (2013) 304 (8), C768-79 DOI: 10.1152/ajpcell.00417.2012
Cited by 200
AI Summary: OriGene products contributed to this study by providing the ATOX1 (NM_005125) expression plasmid, which was used to manipulate copper chaperone levels and assess their role in cellular copper uptake.
Snippet: Relative Western blot band intensity, and thus relative expression, were determined using Quantity One Software (Bio-Rad, Hercules, CA). ... NM_005125) were obtained from Origene (Rockville, MD) and Genecopia (Rockville, MD), respectively.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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