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CLPP (NM_006012) Human Over-expression Lysate

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SKU
LC416925
Lyophilized CLPP HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200301
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006012, NP_006003
Synonyms DFNB81; PRLTS3
Predicted MW 30.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006003
Locus ID 8192
Cytogenetics 19p13.3
Protein Families WB
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Other "CLPP" proteins (3)
SKU Description Size Price
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TP300301 Recombinant protein of human ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli) (CLPP), nuclear gene encoding mitochondrial protein, 20 µg 20 ug
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file-alt Citations

LACE1 interacts with p53 and mediates its mitochondrial translocation and apoptosis
Cesnekova, Spacilova, Hansikova et al
Oncotarget (2016) 7 (30), 47687-47698 DOI: 10.18632/oncotarget.9959
Snippet: To find out whether overexpression of wild-type LACE1-FLAG (NM_145315) leads to correct mitochondrial targeting of the fusion protein, we have transiently transfected wild-type HEK293 cells with a C-FLAG-fusion human LACE1 ORF construct (OriGene Technologies). The cells were harvested 48 hours post-transfection and used to prepare mitochondrial fractions
FigureFigureFigure
Showing 1-1 of 1 papers.
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