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ARID1A (NM_006015) Human Over-expression Lysate

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SKU
LC416884
Lyophilized ARID1A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218151
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006015, NP_006006
Synonyms B120; BAF250; BAF250a; BM029; C1orf4; CSS2; ELD; hELD; hOSA1; MRD14; OSA1; P270; SMARCF1
Predicted MW 241.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006006
Locus ID 8289
Cytogenetics 1p36.11
Protein Families WB
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Other "ARID1A" proteins (2)
SKU Description Size Price
LY416884 Transient overexpression lysate of AT rich interactive domain 1A (SWI-like) (ARID1A), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP762286 Purified recombinant protein of Human AT rich interactive domain 1A (SWI-like) (ARID1A), transcript variant 1, Asn1986-End, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Role of ARID1A in epithelial‑mesenchymal transition in breast cancer and its effect on cell sensitivity to 5‑FU
Wang, Gao, Zhou et al
International journal of molecular medicine (2020) 46 (5), 1683-1694 DOI: 10.3892/ijmm.2020.4727
Cited by 13
AI Summary: OriGene’s pcMV6-XL4-ARID1A overexpression vector (cat. no. SC303719) and the control pcMV6-XL4 vector were used to manipulate ARID1A expression in breast cancer cells, enabling the study to assess ARID1A’s role in epithelial-mesenchymal transition and sensitivity to 5-FU.
Snippet: cruz Biotechnology, Inc. The pcMV6‑XL4 and pcMV6‑XL4‑ARId1A (cat. no. Sc303719) vectors were obtained from OriGene Technologies, Inc., and pcMV6‑XL4 was used as a nega‑ tive control (Nc). For transfection, the McF7
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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