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CLN5 (NM_006493) Human Over-expression Lysate

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SKU
LC416612
Lyophilized CLN5 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214709
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006493, NP_006484
Predicted MW 46.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006484
Locus ID 1203
Cytogenetics 13q22.3
Protein Families WB
Protein Pathways Lysosome
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Other "CLN5" proteins (3)
SKU Description Size Price
PH314709 CLN5 MS Standard C13 and N15-labeled recombinant protein (NP_006484) 10 ug
$3,360.00
LY416612 Transient overexpression lysate of ceroid-lipofuscinosis, neuronal 5 (CLN5) (as WB positive control) 100 ug
$436.00
TP314709 Recombinant protein of human ceroid-lipofuscinosis, neuronal 5 (CLN5), 20 µg 20 ug
$867.00

file-alt Citations

Knockdown of CLN5 inhibits the tumorigenic properties of glioblastoma cells via the Akt/mTOR signaling pathway
Xing, Li, Zhao
Oncol Lett (2021) 21 (5), 387 DOI: 10.3892/ol.2021.12648
Cited by 6
AI Summary: OriGene products, specifically the si-CLN5 and negative control (NC) siRNA, were used to knock down CLN5 expression in glioblastoma cell lines, enabling the study of its effects on tumorigenic properties and signaling pathways.
Snippet: The small interfering (si)-CLN5 (OriGene Technologies, Inc.) sequence was 5′-CCTTATTGTCAAGCTAAGT-3′ and the negative control (NC) siRNA (OriGene Technologies, Inc.) sequence was 5′-GGCUGUAUGAGCACCGUUATT-3′. A total of 5 µl liposome was;- The small interfering (si)‑CLN5 (OriGene Technologies, Inc.) sequence was 5'‑CCT TAT TGT CAA GCT AAG T‑3' and the negative control (NC) siRNA (OriGene Technologies, Inc.) sequence was 5'‑GGC UGU AUG AGC ACC GUU ATT‑3'. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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