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TLK2 (NM_006852) Human Over-expression Lysate

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SKU
LC416375
Lyophilized TLK2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218935
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006852, NP_006843
Synonyms HsHPK; MRD57; PKU-ALPHA
Predicted MW 85.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006843
Locus ID 11011
Cytogenetics 17q23.2
Protein Families WB
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Other "TLK2" proteins (3)
SKU Description Size Price
PH318935 TLK2 MS Standard C13 and N15-labeled recombinant protein (NP_006843) 10 ug
$3,360.00
LY416375 Transient overexpression lysate of tousled-like kinase 2 (TLK2), transcript variant A (as WB positive control) 100 ug
$665.00
TP318935 Recombinant protein of human tousled-like kinase 2 (TLK2), transcript variant A, 20 µg 20 ug
$867.00

file-alt Citations

Amplification of TLK2 Induces Genomic Instability via Impairing the G2-M Checkpoint
Kim, Anurag, Veeraraghavan et al
Mol. Cancer Res. (2016) 14 (10), 920-927 DOI: 10.1158/1541-7786.MCR-16-0161
AI Summary: OriGene's full-length cDNA of TLK2 (SC115810) was used in this study to investigate the effects of TLK2 overexpression on genomic instability and the G2-M checkpoint in aggressive breast cancer cells.
Snippet: ...Alexa 488 goat anti-rabbit antibody (Invitrogen) for 1 hour...Roche). Following primary antibodies were used for Western blot...S10), and pAurora kinases antibodies were purchased from Cell Signaling...full-length cDNA of TLK2 (Origene, SC115810), the open reading...
FigureFigure
Showing 1-1 of 1 papers.
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