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ABCB8 (NM_007188) Human Over-expression Lysate

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SKU
LC416139
Lyophilized ABCB8 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224948
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_007188, NP_009119
Synonyms EST328128; M-ABC1; MABC1; MITOSUR
Predicted MW 77.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_009119
Locus ID 11194
Cytogenetics 7q36.1
Protein Families WB
Protein Pathways ABC transporters
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Other "ABCB8" proteins (2)
SKU Description Size Price
LY416139 Transient overexpression lysate of ATP-binding cassette, sub-family B (MDR/TAP), member 8 (ABCB8), nuclear gene encoding mitochondrial protein (as WB positive control) 100 ug
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TP762378 Purified recombinant protein of Human ATP-binding cassette, sub-family B (MDR/TAP), member 8 (ABCB8), nuclear gene encoding mitochondrial protein, Leu5-Leu124, with N-terminal His-ABP tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Identification of an ATP-sensitive potassium channel in mitochondria
Paggio, Checchetto, Campo et al
Nature (2019) 572 (7771), 609-613 DOI: 10.1038/s41586-019-1498-3
AI Summary: In this study, OriGene products including the MITOK (OriGene Cat# RC208444) and MITOSUR (OriGene Cat# RC207553) cDNA clones were used to overexpress and characterize the mitochondrial ATP-sensitive potassium channel subunits, enabling the identification and functional analysis of mitoKATP channels in mitochondrial physiology.
Snippet: Page 1. Article https://doi.org/10.1038/s41586-019-1498-3 Identification of an ATP-sensitive potassium channel in mitochondria Angela Paggio1,4, Vanessa checchetto2,4, Antonio campo1, roberta Menabo3, Giulia Di Marco1;- The pore-forming and ATP-binding subunits of a mitochondrial protein complex that mediates ATP-dependent potassium currents are identified and characterized, revealing the role of ...
Showing 1-1 of 1 papers.
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