B3GNT3 (NM_014256) Human Over-expression Lysate
SKU
LC415403
Lyophilized B3GNT3 HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC209669 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_014256, NP_055071 |
| Synonyms | B3GAL-T8; B3GN-T3; B3GNT-3; beta3Gn-T3; HP10328; TMEM3 |
| Predicted MW | 42.5 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_055071 |
| Locus ID | 10331 |
| Cytogenetics | 19p13.11 |
| Protein Families | WB |
| Protein Pathways | Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "B3GNT3" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LY415403 | Transient overexpression lysate of UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 (B3GNT3) (as WB positive control) | 100 ug |
$436.00
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Citations
B3GNT3: A prognostic biomarker associated with immune cell infiltration in pancreatic adenocarcinoma
Kong, Zhao, Xia et al
Oncol Lett (2021) 21 (2), 159 DOI: 10.3892/ol.2020.12420
Kong, Zhao, Xia et al
Oncol Lett (2021) 21 (2), 159 DOI: 10.3892/ol.2020.12420
Cited by 9
AI Summary: In this study, OriGene products including the B3GNT3 antibody (Cat# TA506514) and B3GNT3 cDNA clone (Cat# RC203307) were used to assess B3GNT3 expression and its prognostic significance in pancreatic adenocarcinoma, contributing to the identification of B3GNT3 as a potential biomarker associated with immune cell infiltration.
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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