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SH3BP4 (NM_014521) Human Over-expression Lysate

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SKU
LC415223
Lyophilized SH3BP4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208730
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014521, NP_055336
Synonyms BOG25; TTP
Predicted MW 107.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_055336
Locus ID 23677
Cytogenetics 2q37.2
Protein Families WB
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Other "SH3BP4" proteins (3)
SKU Description Size Price
PH308730 SH3BP4 MS Standard C13 and N15-labeled recombinant protein (NP_055336) 10 ug
$3,360.00
LY415223 Transient overexpression lysate of SH3-domain binding protein 4 (SH3BP4) (as WB positive control) 100 ug
$436.00
TP308730 Recombinant protein of human SH3-domain binding protein 4 (SH3BP4), 20 µg 20 ug
$867.00

file-alt Citations

DNA and RNA sequencing identified a novel oncogene VPS35 in liver hepatocellular carcinoma
Zhang, Tang, Liang et al
Oncogene (2020)
Cited by 42
AI Summary: OriGene products pCMV6-XL5-VPS35 (Cat# SC113646), pCMV6-XL4-SH3BP4 (Cat# SC108606), and pCMV6-Entry-PNO1 (Cat# RC203434) provided the full-length cDNAs of VPS35, SH3BP4, and PNO1, which were essential for validating the oncogenic functions of these genes in liver hepatocellular carcinoma in this study.
Snippet: Liver hepatocellular carcinoma (LIHC) is the second leading cause of cancer mortality worldwide. Although cancer driver genes identified so far have been considered to be saturated or nearly saturated, challenges remain in discovering novel genes underlying;The full-length cDNA of VPS35 (Accession No. NM_018206) was amplified from plasmid pCMV6-XL5-VPS35 (Cat# SC113646, OriGene, Rockville, MD), and inserted into the BamH I and Xho I sites of the shuttle vector pAdTrack-TO4 (from Dr T-C He, University of Chicago, Chicago, IL). The cDNAs of SH3BP4 (NM_014521) and PNO1 (NM_020143) were amplified from plasmid pCMV6-XL4-SH3BP4 (Cat# SC108606, OriGene) and pCMV6-Entry-PNO1 (Cat# RC203434),
Showing 1-1 of 1 papers.
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