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ZNF423 (NM_015069) Human Over-expression Lysate

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SKU
LC414807
Lyophilized ZNF423 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218703
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015069, NP_055884
Synonyms Ebfaz; hOAZ; JBTS19; NPHP14; OAZ; Roaz; Zfp104; ZFP423
Predicted MW 144.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_055884
Locus ID 23090
Cytogenetics 16q12.1
Protein Families WB
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Documents
Resources
Other "ZNF423" proteins (1)
SKU Description Size Price
LY414807 Transient overexpression lysate of zinc finger protein 423 (ZNF423) (as WB positive control) 100 ug
$665.00

file-alt Citations

ZNF423 modulates the AMP-activated protein kinase pathway and metformin response in a single nucleotide polymorphisms, estrogen and selective estrogen receptor modulator dependent fashion
Qin, Ingle, Kim et al
Pharmacogenet Genomics (2021) 31 (7), 155-164 DOI: 10.1097/FPC.0000000000000435
AI Summary: OriGene products, specifically the ZNF423 shRNA (OriGene, TL313755) and ZNF423 cDNA (OriGene, SC119551), were used in this study to modulate ZNF423 expression in breast cancer cells, enabling the authors to investigate the gene's role in AMPK signaling and cellular response to metformin and SERMs.
FigureFigureFigure
4-Hydroxytamoxifen enhances sensitivity of estrogen receptor α-positive breast cancer to docetaxel in an estrogen and ZNF423 SNP-dependent fashion
Wang, Qin, Zayas et al
Breast Cancer Res. Treat. (2019)
AI Summary: OriGene products contributed to this study by providing the VRK1 and PBK plasmids, which were used to transiently overexpress these genes in ZNF423 knockdown cells for rescue experiments.
Snippet: For rescue experiments, cells were reverse transfected with ZNF423 siRNA or control siRNA and allowed to adhere to the plate. Twelve hours later, ZNF423 knockdown cells were transiently overexpressed with the VRK1 or PBK plasmids (Origene, Rockville, MD)
Breast cancer chemoprevention pharmacogenomics: Deep sequencing and functional genomics of the ZNF423 and CTSO genes
Liu, Ho, Schaid et al
NPJ Breast Cancer (2017) 3, 30 DOI: 10.1038/s41523-017-0036-4
AI Summary: OriGene’s wild type human ZNF423 (NM_015069) and CTSO (NM_001334) cDNA products were used as templates for site-directed mutagenesis and functional studies to assess the impact of genetic variants on gene expression and protein function in this breast cancer chemoprevention pharmacogenomics study.
Snippet: …The wild type (WT) human ZNF423 (NM_015069) and CTSO (NM_001334). … (Rockville, Maryland,. ...;The wild type (WT) human ZNF423 (NM_015069) and CTSO (NM_001334) cDNAs were obtained from OriGene Technologies, Inc. …
FigureFigureFigure
Showing 1-3 of 3 papers.
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