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MNK2 (MKNK2) (NM_017572) Human Over-expression Lysate

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SKU
LC413712
Lyophilized MKNK2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210081
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_017572, NP_060042
Synonyms GPRK7; MNK2
Predicted MW 46.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_060042
Locus ID 2872
Cytogenetics 19p13.3
Protein Families WB
Protein Pathways Insulin signaling pathway, MAPK signaling pathway
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file-alt Citations

Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays
Forsström, Bisławska Axnas, Stengele et al
Mol. Cell Proteomics (2014)
AI Summary: OriGene products, specifically the recombinant proteins MKNK2 (LY413712), RNF214 (LY403969), GPR56 (LY428998), PRY2 (LY424151), CDK2AP2 (LY417039), MS4A8B (LY403116), and STARD13 (LY406044), were used in this study to validate antibody binding and specificity observed in peptide array experiments.
Snippet: MKNK2 LY413712, RNF214 LY403969, GPR56 LY428998, PRY2 LY424151, CDK2AP2 LY417039, MS4A8B LY403116, STARD13 LY406044, all from Origene, Rockville, MD) were run on precast 4-20% CriterionTM TGXTM SDS-PAGE gradient gels (Bio-Rad ... Cite SaveSaving
Showing 1-1 of 1 papers.
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