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ASAP1 (NM_018482) Human Over-expression Lysate

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SKU
LC413020
Lyophilized ASAP1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217979
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_018482, NP_060952
Synonyms AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P
Predicted MW 125.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_060952
Locus ID 50807
Cytogenetics 8q24.21-q24.22
Protein Families WB
Protein Pathways Endocytosis, Fc gamma R-mediated phagocytosis
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Other "ASAP1" proteins (1)
SKU Description Size Price
LY413020 Transient overexpression lysate of ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) (as WB positive control) 100 ug
$665.00

file-alt Citations

Antibodies Directed against a Peptide Epitope of a Klebsiella pneumoniae-Derived Protein Are Present in Ankylosing Spondylitis
Puccetti, Dolcino, Tinazzi et al
PLoS ONE (2017) 12 (1), e0171073 DOI: 10.1371/journal.pone.0171073
AI Summary: OriGene’s recombinant ASAP1 protein was used in immunoblot assays to test antibody binding, helping to demonstrate the presence of specific autoantibodies in ankylosing spondylitis patients.
Snippet: c) Western Blotting. The antibody binding to the Arf GAP with the SH3 Domain, Ankyrin Repeat and PH Domain (ASAP1) protein was tested using the recombinant ASAP1, (OriGene Inc., Rockville, MD, USA) using an immunoblot assay. ... All 7 versions Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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