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SPHK1 (NM_021972) Human Over-expression Lysate

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SKU
LC411847
Lyophilized SPHK1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203398
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_021972, NP_068807
Synonyms SPHK
Predicted MW 43.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_068807
Locus ID 8877
Cytogenetics 17q25.1
Protein Families WB
Protein Pathways Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
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Other "SPHK1" proteins (5)
SKU Description Size Price
PH303398 SPHK1 MS Standard C13 and N15-labeled recombinant protein (NP_068807) 10 ug
$3,360.00
LC405282 Lyophilized SPHK1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY405282 Transient overexpression lysate of sphingosine kinase 1 (SPHK1), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY411847 Transient overexpression lysate of sphingosine kinase 1 (SPHK1), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP303398 Recombinant protein of human sphingosine kinase 1 (SPHK1), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Peretinoin, an acyclic retinoid, inhibits hepatocarcinogenesis by suppressing sphingosine kinase 1 expression in vitro and in vivo
Funaki, Kitabayashi, Shimakami et al
Sci Rep (2017) 7 (1), 16978 DOI: 10.1038/s41598-017-17285-2
AI Summary: OriGene contributed to this study by providing the mammalian expression plasmids for N-terminal myc-FLAG-tagged human SPHK1 and Sp1, which were used to investigate the molecular mechanisms by which peretinoin suppresses SPHK1 expression in liver cancer cells.
Snippet: A mammalian expression plasmid of N-terminal myc-FLAG-tagged human SPHK1 (GenBank data base accession number NM_021972) and one encoding Sp1 under the control of cytomegalovirus promoter were purchased from OriGene (Rockville, MD)
FigureFigureFigure
Showing 1-1 of 1 papers.
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