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GBP5 (NM_052942) Human Over-expression Lysate

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SKU
LC409377
Lyophilized GBP5 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206627
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_052942, NP_443174
Synonyms GBP-5
Predicted MW 66.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_443174
Locus ID 115362
Cytogenetics 1p22.2
Protein Families WB
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Other "GBP5" proteins (7)
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LC427458 Lyophilized GBP5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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TP306627 Recombinant protein of human guanylate binding protein 5 (GBP5), transcript variant 1, 20 µg 20 ug
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file-alt Citations

Regulation of Synovial Inflammation and Tissue Destruction by Guanylate Binding Protein 5 in Synovial Fibroblasts From Patients With Rheumatoid Arthritis and Rats With Adjuvant-Induced Arthritis
Haque, Singh, Ouseph et al
Arthritis Rheumatol (2021) 73 (6), 943-954 DOI: 10.1002/art.41611
Cited by 34
AI Summary: In this study, OriGene products including human GBP-5 cDNA (RC208674) and GBP-5-specific siRNA (SR320431) were used to modulate GBP-5 expression in synovial fibroblasts, enabling the authors to investigate the regulatory role of GBP-5 in synovial inflammation and tissue destruction in rheumatoid arthritis.
FigureFigure
Showing 1-1 of 1 papers.
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